FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU180-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU180-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87179749
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG113850.027.0402055
GTCTAGG255950.022.7773421
TATACCG272600.021.471035
TAGAGCG257900.021.1106365
GTCTATG307850.019.6009961
GTACCGT175400.019.1224216
CCTAGAC258400.018.9202443
ATAGACG140000.018.4957684
AGTACCG236350.018.2916895
CACTGAG392500.018.0794737
ACTGAGC377850.017.9253658
GGACTGC222000.017.483846
GACGGTC163350.017.3010587
GTCTTCT2095450.017.2640994-95
GTGTATG367550.017.10241
CTTCTGC1441300.017.05822894-95
CCGTCTT3273550.017.04592394-95
TATACTG373650.016.9612885
GTATAAG392000.016.8477691
TCTTCTG1796600.016.6366594-95
Produced by FastQC (version 0.11.5)