FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU180-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU180-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135266885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT5441350.025.06924494-95
TCTGCTT3849400.023.7364394-95
CTTCTGC7494550.022.96837694-95
TAGACGG152050.020.9029225
TATACCG322150.020.5870725
TCTTCTG9764950.020.36600794-95
CTGCTTG2527600.019.99149794-95
GTCTAGG311650.019.8499661
GTACCGT226150.018.7595316
GTCTTCT11854150.018.02344994-95
GTCTATG367100.017.4211431
TAGAGCG338300.017.1607595
ACTGTCC226400.017.098148
GTATAAG448550.016.8529241
CACTGAG495850.016.6867837
ACTGAGC496750.016.6849678
TGCTTGA1720350.016.62300394-95
CTAGACA370800.016.3115184
TACCGTG291300.016.3055087
AGTACCG313600.016.255155
Produced by FastQC (version 0.11.5)