FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU15-W-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU15-W-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100914883
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG254450.019.3266531
CCTAGAC266500.017.3113373
TATACTG337900.017.2513625
GTCTTCT1581900.017.1425694-95
TATACCG218700.017.0742175
GTATAAG326450.016.9270691
TACACCG259850.016.710545
CCGTCTT2701050.016.66605894-95
TAGACGG144550.016.6302765
GTCTATG293450.016.5476191
ACTGTCC197200.016.3782838
GTGTATG318300.016.255861
GTATAGG317100.016.1825241
GTACCGT196000.015.9733556
CTATACT344950.015.9362394
TACACAG217350.015.7376515
TCTTCTG1419950.015.6053494-95
CTAGACA301300.015.5484244
CGTCTTC2424150.015.4434494-95
TCTAGAC251600.015.3717173
Produced by FastQC (version 0.11.5)