FastQCFastQC Report
Sat 3 Oct 2020
HV5V5DRXX_n01_AZU15-S-R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5V5DRXX_n01_AZU15-S-R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136904437
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT4339400.023.90973794-95
CTTCTGC5798150.022.30952394-95
TCTGCTT3171800.021.88620294-95
GTCTAGG339250.021.0928941
TATACCG370750.019.6317045
TCTTCTG7620650.019.49104194-95
CTGCTTG2049400.018.58714994-95
GTACCGT219700.018.575636
GTCTTCT9135950.017.65552394-95
ACTGTCC230600.017.487038
TAGACGG202350.016.9987015
AGTACCG299100.016.8689675
GGACTGC304950.016.7790536
GTATAAG581450.016.7358821
CCTAGAC360500.016.646313
TAGAGCG326350.016.5813735
GTGTATG487050.016.4090521
TACCGTG292550.016.0248057
ACTGAGC440250.015.9240898
TACACCG293600.015.9228015
Produced by FastQC (version 0.11.5)