FastQCFastQC Report
Wed 22 Nov 2017
HV5NCAFXX_n02_dropseq12hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5NCAFXX_n02_dropseq12hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116534587
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3267880.28042146834913484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1601300.051.412471
GTATCAA5036550.045.0022471
TATCAAC7312000.031.0479012
TCAACGC7290000.030.9307674
ATCAACG7369650.030.6836783
CAACGCA7681350.029.3899945
AACGCAG8216500.027.5409226
ACGCAGA9097950.024.8264717
CGCAGAG9147450.024.6437918
CAGAGTG5705400.023.80776610
GCAGAGT9571200.023.498019
AGAGTGA5833150.023.26681311
AGAGTAC4104550.021.58743711
CAGAGTA4239500.021.09143810
GAGTGAA6588900.020.52717612
AGTGAAT6590700.020.3887913
GAGTACG4667700.018.5584412
AGTACGT4719950.018.2708113
GTGAATG7461800.017.93058814
TGAATGG7475400.017.75282315
Produced by FastQC (version 0.11.2)