FastQCFastQC Report
Wed 22 Nov 2017
HV5NCAFXX_n01_dropseq12hpf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5NCAFXX_n01_dropseq12hpf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116534587
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1716900.051.5389941
GTATCAA5197450.043.8691751
TATCAAC7714350.030.1544912
TCAACGC7792550.029.6710784
ATCAACG7829700.029.5730553
CAACGCA8237100.028.082725
AACGCAG8832100.026.2114816
ACGCAGA9849500.023.444987
CGCAGAG9908900.023.2645178
CAGAGTG5646350.022.48643110
GCAGAGT10402450.022.1273579
AGAGTGA5752800.022.01219611
AGAGTAC5014950.020.4654311
CAGAGTA5158000.020.02488110
GAGTGAA6469300.019.52788412
AGTGAAT6508750.019.28220613
GAGTACG5768550.017.42417712
AGTACGT5847600.017.15015813
GTGAATG7367800.017.00325814
TGAATGG7427800.016.76960215
Produced by FastQC (version 0.11.2)