FastQCFastQC Report
Thu 29 Aug 2024
HV5CNBGXV_n01_DO126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5CNBGXV_n01_DO126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779247
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1405821.3041912853467408TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC612990.5686760865578088TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTC423230.39263410514667674TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGG318220.29521542645789633TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTTG304740.28270991470925566TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGG296500.2750655959548937TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTGG283550.263051769757201TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGC262420.24344928732034807TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATTC262300.24333796228994475TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTGG250910.2327713614874954TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGC247310.2294316105753955TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGC245470.22772462677587776TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATGG238210.2209894624364763TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTTG218800.20298263876873776TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTGG211600.19630313694453796TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATTG207250.19226760459241726TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTGC205760.19088531879824258TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTTG203760.18902990162485378TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATTG200940.1864137634103755TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATTG190350.17658932947728168TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGTTGG176570.16380550515263265TruSeq Adapter, Index 3 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTTG175050.16239538810085716TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTTG159160.1476540986582829TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTGG158620.1471531360214679TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATTG157490.14610482531850325TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATGG152190.14118796980902285TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGTTGC149360.13856255450867763TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATTC146410.13582581417792913TruSeq Adapter, Index 3 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTTTGG142210.1319294381138126TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATGG136280.12642812619471472TruSeq Adapter, Index 3 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTTC134970.12521282794614505TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTTTTG129720.12034235786599935TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTTTTC125700.11661296934748781TruSeq Adapter, Index 3 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGGATTC117880.1093582881995375TruSeq Adapter, Index 3 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTGG115710.10734516056641061TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTTG114690.10639889780798231TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATTG111240.10319830318388659TruSeq Adapter, Index 3 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGTATGG108980.10110168177795721TruSeq Adapter, Index 3 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)