FastQCFastQC Report
Thu 29 Aug 2024
HV5CNBGXV_n01_DO125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV5CNBGXV_n01_DO125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17498504
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC467370.2670914039280158TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTGG362800.20733200963922402TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC339980.19429089481020775TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATGG232570.13290850463559628TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTGG213790.12217615860190105TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTTG206550.11803866204790991TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG200270.11444978382151984TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTTTGG195790.11188956495938167TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC194760.11130094321205972TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGG177320.10133437692730761TruSeq Adapter, Index 2 (97% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)