FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22410221
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14383476.418263345104896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT31450.013.4604594
CCCGTAT8500.011.1191011
CTCCGAT24700.010.7706381
CCCGAAT17500.010.2013341
CGCTAGT4501.9122308E-610.1124331
CGTTCGA11300.09.912003544
CGATTGG12550.08.920754
CGCGTGT4351.4791366E-48.8517321
GTCGGAT10850.08.7108161
CTCGTTT25200.08.6122371
GTCCGAC11000.08.5920321
CTCCTAT21950.08.2926561
CCGGAAA15500.08.13009551
TTGAAAA61550.08.12945218
CCCGTTT21100.08.12902451
GCGTTCG13900.08.05758243
CTCGATT10454.5474735E-118.0393291
CCGATCG4804.3085712E-48.0176943
TACGATA10505.0931703E-117.998474659
CCCGCAT19600.07.858171
Produced by FastQC (version 0.11.5)