FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb162.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb162.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28649733
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326070.1138125789863382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT30450.011.7281481
CGTATTA9700.010.8225493
GTCGCGA8400.010.420231
CTCCTAT64800.010.3198861
CTCGTAT53550.09.9380381
CCCGTTT59950.09.6947241
TCCGCTA14150.09.6449072
CTCGTTT74800.09.5487191
CTCCGTT80250.09.5110411
CGGGTAT21750.09.49751
GTCCGGA48100.09.4626951
CTCCGGT87100.09.4463991
GTCCGTT51550.09.3048311
TCGTATC39850.09.1326092
CTCCGCT106100.09.1077331
CCCGAAT59300.09.0924851
GTCCGCA50800.08.9597561
GCGTTCG40300.08.94500243
CCCGTCT75550.08.8051291
CGTACGA27000.08.68680624
Produced by FastQC (version 0.11.5)