FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26556079
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302370.11386093556959218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38650.038.48901748
ACGTCCG40150.038.1071232
CGTCCGC49900.030.80149333
TCGTATG48300.030.79749945
CGTATGC50050.029.51192546
GTCACGT64700.023.86402729
GTCCGCA70150.022.05963334
GTATGCC69800.021.86369347
CTCGTAT68200.021.55420544
AGTCACG71700.021.24094428
ACACGTC75350.021.03827313
CGCACAT69750.020.97889337
ATGCCGT76450.020.00807849
ACGTCTG83350.019.1449915
TCACGTC83200.018.5999830
CGTCTGA86050.018.5036816
ATCGGAA86600.017.618622
GATCGGA89200.017.2710721
TGCCGTC91200.016.77218650
CACGTCT96250.016.76085314
Produced by FastQC (version 0.11.5)