FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31687322
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391920.12368353501125781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32900.028.83265948
CGTCCCG44100.020.80051435
TCGTATG46300.020.25962845
ACCCGTC45650.019.86529532
CGTATGC48650.019.42590346
GTATGCC70500.013.75294747
CTCGTAT69600.012.7729944
ATGCCGT76900.012.60863749
GTCCCGA81900.011.58450636
CGTATTA12850.011.1651493
TGCCGTC89400.011.04152450
ACGTCTG90950.010.92711715
CACCCGT92450.010.90742631
CCCGTAT30850.010.8945741
ACGTACG15550.010.80430353
ACACGTC92300.010.69133413
TCTCGTA82900.010.554419543
CGTCTGA95500.010.47990716
GCCGTCT96550.010.36878351
TCACCCG103900.09.87383830
Produced by FastQC (version 0.11.5)