FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb159.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb159.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27549856
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354350.12862136194105697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28800.012.765011
TATGCCG19500.010.76982248
GTCCGGT38800.010.73841
CGCCTAT12400.09.8825891
CTCCGAT67100.09.4967111
CTCCTAT65650.09.2798051
GTCCGTT44600.09.2634241
CCCGTTT60200.08.724071
GTCCGTA25000.08.68312551
GTCCCGT45900.08.619661
CTCGTTT71600.08.5575491
CGCTACT19250.08.5485311
CGCTAGT9504.9112714E-118.4767341
CGCTAGA12700.08.2706931
CTCGTAT50250.08.2218651
CTCCGCT95550.08.1714391
TCGCATT24900.08.1512092
TCCGCTA15550.08.10150052
GTCGCGA6101.01740625E-58.0356791
CCGTATC28750.08.03340152
Produced by FastQC (version 0.11.5)