FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb158.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb158.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31951655
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG327910.10262692182924484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG57750.012.30248243
CGTTCGA59400.011.72541444
CGTATTA11950.011.7132173
AAGTGCG63150.011.13990639
CCCGTAT33200.010.6512661
AGTGTCG70400.010.59054151
ACGTACG13450.010.40927153
CGCAAGT70150.010.33067436
GTCCGGT46300.09.9062261
GTTCGAA73000.09.87670445
AGTGCGT73750.09.72819640
CTCCGAT74500.09.5402041
GTGCGTT76250.09.50109741
TCGCATT29300.08.8380472
CCCGTAC34350.08.7657611
GTCGCAT25750.08.7020321
GTCCGTT53600.08.5570571
AACCCGG86500.08.37802326
TCCGCTA16900.08.28257752
GAACCCG88050.08.23015325
Produced by FastQC (version 0.11.5)