FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb157.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb157.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33535801
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG417040.1243566539531887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31850.023.29848748
TCGTATG42550.017.35629545
CGTATGC43150.017.2778846
TGTTACG34150.012.1938913
TACGACT37300.011.0701966
ACGTACG18150.010.99223353
GTATGCC73700.010.54340747
CTCGTAT69400.010.48982644
CCCGTAT35100.010.4735121
GTTACGA42800.010.4652354
TCGACCG43450.09.75018431
GTCCCGT61050.09.1758111
GTCCGTA28300.09.1549391
ACGTCTG88700.09.11342315
GTCCGGT47800.09.0824691
ATGCCGT84050.08.99549649
GTCCGTT50800.08.9596241
TTACGAC48100.08.9483355
ACACGTC88100.08.77816413
CGTATTA12450.08.7132143
Produced by FastQC (version 0.11.5)