FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb156.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb156.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29804626
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG12150.010.94678553
CCCGTAT30350.010.1515641
CGTTCGA30750.010.12978244
CTCGTAT54400.010.0400221
CTCGCGT13700.09.4556231
GTCCGGT42600.09.4514241
CTCCGAT69600.09.2055731
CCCGAAT54950.09.0475221
GCGTTCG33900.08.9818443
GTCGCAT25800.08.8206921
TCGTATC42150.08.6343812
CTCCTAT65300.08.5249721
CTCCGTT78500.08.2956861
CGTATTA10202.7284841E-118.2337663
CTCCGGT93200.08.1893521
GTCCCGT50100.08.036541
TCGCGTT14850.08.0121292
TACGAAA18450.07.96704356
CCGTATT38900.07.91641472
AAGTGCG39500.07.88582939
Produced by FastQC (version 0.11.5)