FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33733784
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA425020.12599238792778183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366740.10871593889378078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA223850.016.4171344
AAGTGCG229600.016.11221539
GCGTTCG228500.016.09797543
CGCAAGT233300.015.891436
AGTGTCG235400.015.68744651
AGTGCGT239400.015.46655940
GTTCGAA240950.015.39748445
ACGTACG27700.015.28915653
GTGCGTT245400.015.15982241
AGGCGTA251550.014.7039478
AACCCGG251300.014.57224826
GAACCCG255700.014.43034425
GCCGCAA260400.014.31927834
CCGCAAG263350.014.06545835
GTGTCGA266600.013.85120752
TACGTAC31750.013.67014852
GATCAAT273500.013.63984960
ACAGGCG277450.013.5709886
GCGTAGC279650.013.33913210
ACCCGGG280600.013.18789127
Produced by FastQC (version 0.11.5)