FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_mb154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_mb154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29693824
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1146540.38612069634412866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39350.035.84795446
TCGTATG48950.029.17130743
CGTATGC50550.027.90321544
TATCTCG52100.026.46775839
ACGCCAA60150.023.92404632
CGCCAAT60200.023.4968533
GTCACGC69150.020.3543829
CTCGTAT74100.019.12849842
CCAATAT76600.018.92263835
GTATGCC75100.018.64228845
ATGCCGT80950.017.7286147
AGTCACG83850.016.99465828
ATATCTC87550.016.19225338
TCTCGTA88550.015.69054241
TCACGCC90650.015.68146430
GCCAATA93700.015.282866534
CGTCTGA97650.014.90778816
ACGTCTG95550.014.86899615
ACACGTC95800.014.86662113
TGCCGTC102150.013.98100148
Produced by FastQC (version 0.11.5)