FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_ba70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_ba70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28885517
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC43150.019.46448360
GCATACG48550.016.94006756
TACGATA49850.016.6377959
CATACGA50100.016.62510757
ATACGAT51500.016.44466858
TATGCCG18750.016.05425346
CGATACT52350.015.84318961
ACGTATA23100.015.30093918
TAAGCCG48300.015.00085846
TCGAATG68900.013.7640422
GACGTAT25450.013.61288217
ATCCGAA61450.013.44477925
CGAATGT74850.013.3242783
CTCCGAT90300.013.1821531
GACTACG27800.013.09774435
AAATCCG63450.013.02006323
CTCGAAT99950.012.8902161
CGTATAG29900.012.87494119
AATCCGA67700.012.82342524
GATATTA69350.012.81710468
Produced by FastQC (version 0.11.5)