FastQCFastQC Report
Sat 27 Oct 2018
HV3T2BGX7_n01_ba69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_ba69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36709288
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG377570.10285407878245963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC56900.016.97491660
CATACGA62450.015.74716757
ATCCGAA87150.015.54583725
GCATACG63150.015.406527556
TACGATA63500.015.21065659
CGATACT65650.014.9257661
ATACGAT66700.014.74348558
TAAGCCG57650.014.57120746
AATCCGA94300.014.18061424
AAATCCG93300.013.9945823
CGTAGTA99500.013.259397566
CTCGAAT129650.012.7464131
CTCGTTT111550.012.5233881
TCGAATG90200.012.3760142
GACGTAT34900.012.33341617
ACGTATA35250.012.31038518
CGAATGT100400.012.0943933
CTCCGAT114950.011.6351741
TGTCGTA119900.011.35373863
CCGGACT77450.011.07249350
Produced by FastQC (version 0.11.5)