FastQCFastQC Report
Fri 26 Oct 2018
HV3T2BGX7_n01_ba68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3T2BGX7_n01_ba68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29554837
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG356050.1204709740067252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43550.042.11459446
TCACCGA68050.029.12179830
TCGTATG63650.027.98763743
CTCGTAT65400.027.07777842
CGTATGC67750.026.75970544
TATCTCG67250.026.07338539
CGATGTA76300.025.88093834
ACCGATG80850.024.94461332
GTCACCG79850.024.73039829
ACACGTC81600.024.48674813
ACGTCTG82350.024.09392415
CGTCTGA83200.023.9319716
CACCGAT83550.023.92900831
GTATGCC78900.023.422145
ATGCCGT79950.023.11578447
CCGATGT87950.022.85133
CACACGT100450.020.3793512
CACGTCT99950.020.23632414
TGCCGTC90700.019.91325248
GATCGGA100700.019.6445221
Produced by FastQC (version 0.11.5)