FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19371575
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11956996.172440805665001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC60900.042.92470627
CCAGTCA65300.041.3719426
CTCCAGT75250.039.8082924
GAACTCC93650.035.3870221
CTGAACT98850.034.09182419
TGAACTC101100.032.8485120
CGTCTGA106050.031.90915716
AACTCCA104700.031.81940522
ACGTCTG110450.030.73312215
CACGTCT118400.028.43308814
ACACGTC124650.027.37240813
TCCCTAC23700.026.14746740
TACACGA27200.026.00069444
CACACGT132750.025.75488312
CCCTACA27100.025.19249241
CGACGCT28000.025.12155348
GCACACG138350.024.81351511
ACTCCAG135500.024.38029523
CTTCCGA27150.024.10344755
CCTACAC28900.023.98672342
Produced by FastQC (version 0.11.5)