FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_9_bira_TGA1_2.5h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_9_bira_TGA1_2.5h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35676681
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG53650.018.4643341
TATGCCG39100.018.26064946
CGTATGC42150.017.02237944
ATGCCGT48300.015.21720647
GTATGCC52850.014.50313545
TGCCGTC52600.014.03975348
GCCGTCT49850.013.90167249
AGTCACG57300.012.1552628
AGGGGGG42400.011.0625671
GTCACGA65100.010.91392229
CGATCAG72100.010.24259433
CTCGTAT68000.010.0881742
TCGTATG88050.08.466701543
TCTCGTA92850.07.91595341
CAGTCAC92150.07.90013327
TCCAGTC103200.07.562935425
CCAGTCA101050.07.23894326
CACGATC115250.07.13659331
ACTCCAG109650.07.11810623
TCACGAT110100.07.08895430
Produced by FastQC (version 0.11.5)