FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_8_ABF3_60_K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_8_ABF3_60_K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27438880
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC286550.045.47348829
ACACTTG288950.045.03489332
CACACTT297550.043.68629531
CAGTCAC300850.043.3937127
TCACACT302050.043.0702130
TGAATCG160050.041.6368337
AGTCACA314250.041.50993728
CCAGTCA321200.040.6115426
CACTTGA328700.039.53561433
CTCCAGT340750.039.1236324
CTTGAAT322900.038.57639335
TTGAATC308900.036.6767836
ACTTGAA353150.036.5108634
TCCAGTC375700.035.42820725
AACTCCA377850.035.31011222
ACGTCTG383850.035.07711815
GAACTCC389350.034.35694521
CTGAACT392200.034.34816719
CACGTCT392300.034.25019514
CGTCTGA394600.034.2278916
Produced by FastQC (version 0.11.5)