FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_7_ABF3_60_K_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_7_ABF3_60_K_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24485286
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC777410.3175008860423358TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC264680.10809757337529159TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC439500.053.80159828
CAGTCAC444300.053.3072227
CGTATGC163100.052.59623344
GTATGCC175450.052.54454845
CCAGTCA458600.051.6139526
CTCGTAT138350.050.59619542
CTCCAGT477050.050.3808824
TCGTATG142100.049.3592943
TCCAGTC496350.048.3160125
TCACCAG491500.048.31567830
GTCACCA496700.047.80290629
CACCAGA494800.047.77416231
ACGTCTG520250.046.72195415
ACCAGAT507600.046.63840532
TATGCCG191000.046.5442146
TCTCGTA149000.046.41641
CGTCTGA523800.046.4052116
TCTGAAC527150.046.03072718
AACTCCA523650.045.85075822
CTGAACT528350.045.83997319
Produced by FastQC (version 0.11.5)