FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_6_ABF3_60_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_6_ABF3_60_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18349396
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1322390.7206722226715255TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC182200.057.1287444
GTATGCC184750.056.4914745
TCGTATG186500.055.39870543
CTCGTAT184550.055.3582242
AGTCACG212450.053.73969728
TCTCGTA196850.051.97046741
TATCTCG203900.051.6843639
CAGTCAC222050.051.40071527
TATGCCG209900.049.5891946
CCAGTCA231650.049.27071426
GCCAATA234050.048.7343834
CTCCAGT251250.046.65272524
ATGCCGT221750.046.1027147
ACTCCAG256150.045.71979523
ATCTCGT231300.045.27403340
CCAATAT255100.044.5347435
CACGCCA257300.044.50774431
CGCCAAT258800.044.3984233
ACGCCAA261150.043.9184832
ATATCTC242900.043.73126638
Produced by FastQC (version 0.11.5)