FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_5_ABF3_60_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_5_ABF3_60_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26503190
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1052680.3971899231752857TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC997160.37624150149472574TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC345980.1305427761714722TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC342700.059.438844
GTATGCC357000.059.2441645
ACACAGT631550.058.2448832
CACAGTG632150.058.06226333
TCGTATG297050.057.6156543
CACACAG642150.057.4196931
GTCACAC643050.057.3290929
ACAGTGA623450.056.83434334
CAGTCAC650600.056.79377727
CAGTGAT587750.056.63617335
AGTCACA654300.056.47775328
TCACACA661750.055.8037430
CCAGTCA661700.055.7931426
ACTCCAG680400.055.00562723
CTCCAGT681050.054.9736824
GAACTCC704200.053.2950921
AACTCCA716550.052.4499922
ACGTCTG721500.052.4436815
CACGTCT722550.052.38684514
CTCGTAT166600.052.1630442
Produced by FastQC (version 0.11.5)