FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_4_ABF2_60_K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_4_ABF2_60_K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22783325
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2009160.881855479830095TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC302650.059.02514344
GTATGCC303400.058.7748945
TCGTATG308700.057.584943
CTCGTAT281700.057.52573442
TCTCGTA290100.053.92979441
CACTGAC349450.053.39366531
TATGCCG334300.053.2058846
CAGTCAC363100.052.58250827
AGTCACT364600.052.3469828
ACTGACC359150.051.8245732
GTCACTG370300.051.4654829
CCAGTCA373900.051.12886426
AATCTCG310000.049.93687439
TCACTGA374500.049.7850430
CTCCAGT390400.049.67629624
ATGCCGT353000.049.54445347
ATCTCGT318000.048.5266240
GACCAAT358250.048.4274135
ACTCCAG401500.048.3116523
CCGTCTT344550.047.6410150
Produced by FastQC (version 0.11.5)