FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_3_ABF2_60_K_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_3_ABF2_60_K_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23196880
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1805130.7781779273764402TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC308480.13298340121602562TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC316500.059.74815445
CACTTAG564450.059.7065231
TTAGGCA552750.059.6774434
CGTATGC307800.059.4249344
TAGGCAT524950.058.7575235
CAGTCAC578200.058.2392327
AGTCACT586850.057.5059328
GTCACTT587250.057.32930429
AGGCATC450300.057.0028536
CCAGTCA590300.056.9503326
CTCGTAT272800.056.92642242
TCGTATG285250.056.736343
CTCCAGT602950.056.39403524
ACTCCAG604800.056.2333523
TCACTTA602800.056.04747430
TATGCCG339100.055.00174746
TCCAGTC618650.054.91749625
GAACTCC632050.053.863621
TCTCGTA282600.053.6395241
ACGTCTG642350.053.5926615
Produced by FastQC (version 0.11.5)