FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_2_ABF2_60_N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_2_ABF2_60_N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22083723
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC756570.34259169072171386TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA289450.054.2308634
GTATGCC152950.053.88971745
CGTATGC144250.053.20950744
AGTCACC301200.052.6155328
CAGTCAC304050.052.18001627
CACCGAT304150.051.84018731
CCGATGT306650.051.41743533
ACCGATG309700.051.01276832
CCAGTCA313300.050.62814326
CTCCAGT325050.049.6921724
TATGCCG162000.049.6263646
ACTCCAG326200.049.4528323
TCCAGTC326450.049.3179125
TCACCGA334650.047.42933330
GTCACCG335900.047.17989329
GAACTCC342400.047.0717521
ACGTCTG346400.047.03296315
CTCGTAT148450.046.6823242
GATGTAT324850.046.56519735
CGTCTGA353400.046.10114316
Produced by FastQC (version 0.11.5)