FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_1_ABF2_60_N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_1_ABF2_60_N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31425303
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1135350.3612852993016487TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC205250.051.56585744
GTATGCC207100.050.86838545
TCGTATG220850.047.27368543
CTCGTAT207400.046.2554842
TATGCCG234500.045.02910246
TCTCGTA221800.041.21689241
CAGTCAC265850.041.00987627
ATGCCGT263800.039.8290147
TCACGAT252350.038.76502235
CCAGTCA283000.038.68517726
CCGTCTT269150.037.75018350
CATCACG287850.037.692833
GTCACAT289750.037.6631829
AGTCACA289450.037.64163628
CTCCAGT303300.037.3771624
ATCACGA279000.037.15709334
ACTCCAG308250.036.83365623
GCCGTCT281300.036.03262749
CTGCTTG265850.035.70477757
TCCAGTC317800.035.42949325
Produced by FastQC (version 0.11.5)