FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_16_input_bira_ABF3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_16_input_bira_ABF3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155487
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431351.3669839235591845No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243690.7722738201741918No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90100.2855343723488641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG49600.039.0952261
AGGGGGG17750.039.044741
TATGCCG5200.034.3269348
CGTATGC5250.034.00000446
ATGCCGT5450.032.110149
GCCGTCT5350.029.4392651
GTATGCC6250.029.12000747
TGCCGTC6050.027.19008850
TTGGGGG9800.024.6444241
CACGTGA8100.021.1725131
GTCACGT8250.019.93908329
CCGTCTT8400.019.58333852
TCTCGTA6950.019.13669643
CTCGTAT7500.019.13333944
TCACGTG9000.018.66637630
TGAAACG9850.017.7662235
CGTGAAA10050.017.76091833
AGTCACG10200.016.81346328
TCGTATG11000.016.22727645
GAAACGA11750.015.19149336
Produced by FastQC (version 0.11.5)