FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_15_input_bira_TGA1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_15_input_bira_TGA1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11505918
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1738261.5107529881579203No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG617200.5364196059801574No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253790.22057344750762176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG138900.032.9377021
AGGGGGG62000.031.7295971
CGTCCGC13350.028.05188433
TATGCCG13250.027.99981548
ATGCCGT13950.027.09659649
TGCCGTC13700.026.82464250
ACGTCCG14750.025.15194132
CGTATGC14550.024.77625346
TCCGCAC15450.024.01247635
GTATGCC15950.023.040547
CCGCACA18100.020.11027736
CACGTCC19400.020.02546931
CGCACAT19100.018.8741437
GTCACGT21750.018.02302729
TTGGGGG29200.017.98161
TCACGTC22750.017.69203630
GCCGTCT21450.017.29607451
GCACATC23400.015.10673938
GTCCGCA25950.014.56618834
AGTCACG26250.014.53342728
Produced by FastQC (version 0.11.5)