FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_14_input_ABF3_60_K.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_14_input_ABF3_60_K.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38987588
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG147700.010.18939470
TCCAGTC270850.09.47186670
GTCACGT136400.08.85248470
CTCCAGT332150.08.80911770
ACTCCAG369350.08.65153370
CACGTAG93300.08.59041770
CCAGTCA238550.08.56828470
CGTCTGA860400.08.22103170
GAACTCC509550.08.10504770
CAGTCAC211200.07.95440670
TCTGAAC751400.07.876491570
TGAACTC626800.07.834099370
ACGTCTG942450.07.81353970
GTCTGAA836650.07.793444670
CACGTCT1090350.07.59788570
CTGAACT651050.07.483164370
ACACGTC1210850.07.243544670
AACTCCA588500.07.12476870
TCACGTA130750.07.12033370
GCACACG1449600.07.04043970