FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_13_input_ABF3_60_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_13_input_ABF3_60_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38170434
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG305550.08.62532770
GAACTCC418350.08.50832670
GTCACCC114800.08.41453570
TCCAGTC228300.08.33980670
CTCCAGT279150.08.19979770
ACGTCTG758450.08.02482470
GTCCGCA33800.07.869719536
CGTCTGA694750.07.83869970
GTCTGAA695150.07.72342270
TGAACTC529200.07.665253670
CTGAACT542550.07.650817470
CACGTCT881250.07.597641570
CCAGTCA197700.07.594751470
CGTCCGC32000.07.54676635
CACCCGT81100.07.466003470
TCTGAAC622450.07.450309870
ACACGTC984700.07.378810470
GCACACG1178300.07.280318370
CAGTCAC178600.07.231157370
AGTCACC154900.07.230386370