FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_11_input_ABF2_60_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_11_input_ABF2_60_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36602941
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG104300.08.9596170
AGTCACG126800.08.88789270
ACTCCAG306550.08.59717670
CGTCTGA683200.08.26833170
GAACTCC419300.08.2636670
TCCAGTC229600.08.216351570
ACGTCTG746200.08.20814370
CAGTCAC184700.08.11033970
CTCCAGT281300.08.06245670
CACGTCT872350.07.867720670
CACGGCT83500.07.67055470
CTGAACT541550.07.593840670
GCACACG1164700.07.464481470
GTCTGAA674250.07.45929770
CCAGTCA206300.07.4477970
ACACGTC967650.07.338814370
TCTGAAC613200.07.243052570
TGAACTC518000.07.074228370
CACACGT1073200.07.060563670
TATGCCG39150.06.88369646