FastQCFastQC Report
Sat 18 Jan 2020
HV3K5BGXC_n01_10_bira_ABF3_2.5h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K5BGXC_n01_10_bira_ABF3_2.5h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35810432
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC100150.015.90088131
TGGGGGG40500.014.9520561
CAGTCAC106700.014.30169927
TCCAGTC111400.014.07526525
CCAGTCA110150.013.88549326
TATGCCG31050.013.30099646
ACTCCAG121650.012.8606123
ACGTCTG119700.012.71915415
CTCCAGT128250.012.41706724
TCACTAG129000.012.23622730
CGTCTGA135450.011.67943916
AGTCACT134350.011.5927428
GTCACTA136800.011.41067429
GCACACG134350.011.41035111
ACTAGCT142500.011.22437232
ACACGTC140950.010.975368513
CACACGT145250.010.65051312
CGTATGC37300.010.6032344
TAGCTTA154450.010.49186334
CACGTCT155500.010.06096614
Produced by FastQC (version 0.11.5)