FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23816719
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG409120117.17785308715277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28350.048.572511
GTTAGTC10550.016.3155271
ACGTCTG11750.013.99374515
GCGCGAT2052.0990235E-413.7086171
GCACACG12600.013.60480211
TTAGTCA12650.013.003742
CGGGGGG98350.012.429751
CACACGT14200.012.31825712
CGGGCAT5551.4551915E-1112.0259161
AGCACAC14700.011.66064610
GGGGTAC7850.011.6348611
CACGTCT14800.011.58263714
CGCGGGA5207.348717E-1011.4842621
ACACGTC15100.011.1207413
AAGGGGG7600.011.04931729
GCGGACT3308.746393E-510.6449491
CCGGGAT10750.010.130031
ATGCCGT14200.010.10247647
GGCGAAC9200.09.9275721
CCCCGCT3207.783667E-49.8798441
Produced by FastQC (version 0.11.5)