FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_48_NxW_C16-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_48_NxW_C16-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6941144
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTT13800.012.9358131
CGCCCAT13250.012.6802551
GGGGCCC2508.159804E-512.6010944
GCCGAAT9350.011.9795641
CCCGCCT8550.011.8722921
GGGGGCG2650.001871416510.56634625
CGCCAAT14250.010.5622451
GGCGCCC4501.915596E-610.11075167
GCCCGAT4854.566573E-710.1038981
GCGCCCG4552.2066379E-69.99964468
GCGGGAT10900.09.6337951
CCCGACT8101.6370905E-119.5069291
TAAACGT19550.09.4893163
CCGCTAT7402.8740033E-109.4602141
CCCGCAC9700.09.3821911
AGGCGTA8605.456968E-129.3604736
CGCGTAT4905.667527E-69.2864541
CGCCCCT3400.00140046959.2654441
GTCGGGT8707.2759576E-129.2536111
CGGGGAG8353.092282E-119.2222911
Produced by FastQC (version 0.11.5)