FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_47_NxW_C16-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_47_NxW_C16-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4983571
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT5600.015.0008661
GCGGGCG2505.2708147E-614.00024614
CCCCACT5550.013.8746761
CGGGCGC2751.4091347E-512.72736915
CGCGCAT2508.161128E-512.6007271
GCGCGAT2356.8501144E-411.9155831
CCCCCGT4155.309994E-811.80814827
CCCGCAT6700.011.4932021
GGGCGCA3358.373607E-611.4925116
GGCGGGC3104.804961E-511.29040813
CCCGTTT10850.010.9683761
GCATGCG4351.2388118E-610.46005920
TCGGGCG3351.0573373E-410.4472127
CTAGTTG10100.010.3955978
CGCGGAT7503.6379788E-1210.267261
TACTCTA11400.010.1321644
CGCGGGC2800.00295810039.99947448
GGGTAAT9550.09.895861
CGCGTAT3551.8973816E-49.8597241
CCCGCCT6059.462383E-99.8352791
Produced by FastQC (version 0.11.5)