FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_46_NxW_C16-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_46_NxW_C16-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12359783
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC5600.013.12523938
CGCCCAT23600.012.3100961
GGGGGAT20450.012.15107461
ATGGGGG22550.011.79550859
CGCGGAT19050.011.391791
GCGCGCA6850.011.24094539
CGGGCAT11400.011.0533261
CGCGTAT9300.010.9146631
TCGCACA22800.010.4384079
CCCGTTT27850.010.1801721
ATCGCAC19700.010.1267198
CGAACGC15050.09.5354813
GCCCGCT5906.7364454E-89.4921221
GTCGGAT53450.09.2335071
CGCGCAC7604.783942E-109.21058340
CGGGAAC48500.09.1655241
CGCGCAT6151.27553E-79.1062631
CGGAAAT60400.09.0982531
CGCGGAC6603.9099177E-89.01571751
GCGGAAT26100.08.9852391
Produced by FastQC (version 0.11.5)