FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_45_NxW_C15-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_45_NxW_C15-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5006179
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT7450.013.6245881
GCGGGGT3254.2429747E-712.9234831
GCCGAAT7800.012.1157661
TAAACGT16400.011.5247523
GGGTCGC3651.7278471E-611.50640747
CCCACGT3752.3893535E-611.2003521
GTCCGCT2500.001150814111.2003521
GCCGGAT11250.010.8892311
CGACCGC3903.824094E-610.7695693
CGCCAAT11750.010.42585851
TCGGGGG6201.3096724E-910.1616092
CCCGCCT7603.6379788E-1210.1318971
GGCGCCC3853.89655E-510.00061325
CGCGTAT4209.259153E-610.0003151
TGCACTA17200.09.97124322
TTAAACG19100.09.8955982
ATCGCAC7453.092282E-119.8657868
GCACTAC18200.09.80809823
GCGAAAT10950.09.5893421
CGGACAT6654.3928594E-99.4739821
Produced by FastQC (version 0.11.5)