FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_44_NxW_C15-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_44_NxW_C15-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9484293
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT3954.4519165E-610.633881
CGCGTAT7301.8189894E-1210.5489061
CCGGCAT12950.010.2712051
ATCGCAC14700.010.2380568
GCCGAAT10950.010.2292421
CCCGTTT20900.010.2162411
ACCGCTA18250.010.1639749
GTCGGAT43150.09.8966141
CGCCCAT17450.09.8289761
GGCGCGC5351.6556805E-79.81309838
CGCCCCT5109.0804497E-79.6087181
TCGCACA17400.09.4539879
CCGGGAT24450.09.4487131
CGCGCGT4451.8374463E-59.4390611
CGCGCAC5603.2163007E-79.37491440
GCGGAAT17650.09.3209621
GGGGAAT20700.09.30036451
CGGGCAC9151.8189894E-129.1811651
CGCGGAT14150.09.1527771
GGGCCCC3500.00182548378.9998713
Produced by FastQC (version 0.11.5)