FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_43_NxW_C15-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_43_NxW_C15-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507370
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC5350.013.7384638
CGCGTAT11900.012.3540071
CGGGCAT14150.012.1211861
GCGCGCA6900.011.15926639
CGCCAAT32250.010.6365761
CCCACAT29850.09.8500951
GGCGCCC10700.09.81271267
CGCGGAT21100.09.7875741
GTCCGCT9300.09.78578951
GCGCCCG10150.09.65480768
GCCGAAT19350.09.4064951
GTCGAAT71700.09.2755551
GCCGGAT29400.09.0483991
ATCGCAC22850.09.0370448
CGCGCAC9001.6370905E-118.94432140
CGCCCAT26550.08.7013151
CCGGGAT38600.08.6147321
GGGTCGC11450.08.55862747
ACGCACC19750.08.5071216
GCGAAAT35650.08.4439261
Produced by FastQC (version 0.11.5)