FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_42_NxW_C14-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_42_NxW_C14-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9778741
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC4052.7266651E-912.96318738
CGCGTAT7400.012.7719281
CGCGCAC4451.1059456E-811.79765540
CGGGCAT11200.011.2514611
CGCCAAT20100.011.1457251
GCCGGAT20700.010.9917651
CCCCGAT6704.3655746E-1110.4491171
CCGGGAT24800.09.8803151
CGCCCAT19050.09.3712951
CGCAAAT16100.09.349041
ATCGCAC14800.09.2226618
GGGTGGC19000.09.21007370
CCCGACT15150.09.011071
CCGGCAT14500.08.9321941
CGCGGAT13750.08.9102481
CCCGAAT17100.08.8023121
GCGGGAT19150.08.7739851
GCGCGCA5754.6240602E-68.52166839
CCCGTTT21450.08.485951
TAAACGT31650.08.4041398
Produced by FastQC (version 0.11.5)