FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_41_NxW_C14-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_41_NxW_C14-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7106436
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC3203.6379788E-1217.5000138
CGCGCAC4200.015.8332340
GCGCGCA4101.4551915E-1114.51220239
CCCGTTT15450.013.5932921
CGCCCAT11650.012.3185561
CCCCCGT4003.1826858E-812.25086927
CCCGTCT13250.011.3593751
CCCGCCT8350.011.3182461
TGCGGGG5005.144102E-911.200479533
CCCACAT16450.011.06469251
CGCGCAG3501.3608043E-511.0008571
CGCGGAT10900.010.91828251
CCCGCAT7700.010.9099411
TAAGCGG12050.010.74651548
CCCGTAT5401.7087586E-810.3711781
GCGCACC5855.460606E-910.17087441
CGGGGAT11800.010.0855311
CCCCGAT5906.2700565E-910.0855311
CGCCAAT15800.09.96913051
GGGGCCC2850.0034215569.8253274
Produced by FastQC (version 0.11.5)