FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_40_NxW_C14-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_40_NxW_C14-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12236380
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC6400.013.1252938
GCGGGCG5501.2732926E-1112.0909314
CCGGCAT19150.010.4188371
GCGCGCA8900.010.22486139
CGCGGAT19300.010.1564941
CGCGAAT17750.010.0573831
CGCGCAC9100.010.00009840
GCCGAAT14600.09.8297871
CGCCCAT24450.09.7351621
GTCCGCT8901.8189894E-129.4391821
CGCGTAT8555.456968E-129.4161821
GTCGCAT15350.09.3495051
TCGGGCG8001.3096724E-109.1872157
CGCCCTT12700.09.0954321
GTCGGAT50950.09.0686551
CGGGCAT15200.08.9811951
GGGGGCG6301.8484388E-78.8885462
CCGGGAT31650.08.7370831
ATCGGGC9657.2759576E-128.7056036
GCGGGAT23450.08.6576151
Produced by FastQC (version 0.11.5)