FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_39_NxW_C13-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_39_NxW_C13-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5523405
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT1503.159525E-416.3349191
GCGGGCG2553.910136E-715.09759266
CGGGCAT6550.013.3600761
CGCGGGC3154.2173524E-612.2218665
CCCCGTT5207.7852746E-1011.4434191
GCCGGCT8600.010.989441
CGCCAAT11550.010.6070911
CCGCGCT4005.1724855E-610.501021
CGGACAT8700.010.0584481
CCGGGGT13300.010.0009721
CGCGGAT7453.092282E-119.866731
TAAACGT15850.09.71703153
CGCCCAT10100.09.7039131
GTCGACT10500.09.6676051
GTAGGAC8403.6379788E-129.5830551
GCCGAAT6603.8544385E-99.5463821
CCGGCAT9250.09.4603781
CCGGGTT17850.09.412681
CGCGTAT3350.00122300819.4038981
CGGGAAT14700.09.2866161
Produced by FastQC (version 0.11.5)