FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_38_NxW_C13-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_38_NxW_C13-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12333757
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT8050.013.04461
CGGGGAT18300.012.4327631
CGGGCAT14700.011.1914381
GCCGAAT14050.010.9617961
CGCGGAT16550.010.3634281
CGCCCAT21200.09.7414031
CCGGCAT20300.09.6560021
CCCCACT17550.09.5734731
CCCGTAT12300.09.3910511
CCCCCCT5301.5333553E-69.2460781
GCCGGAT27300.09.2315631
CGCCAAT26900.09.1085891
ATCGGGC9303.6379788E-129.03307156
CGGCAAT26750.09.0288131
CCCGTTT28050.08.9847291
CCGGGGT28500.08.9656831
CGGGAAT33700.08.9325191
GCGGGCC3955.534095E-48.8615221
CCCGAAT20600.08.83571151
CCCCGAT10350.08.7930261
Produced by FastQC (version 0.11.5)