FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_37_NxW_C13-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_37_NxW_C13-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11944876
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT7900.014.6208611
CGGGCAT12950.011.89238551
CGCCCTT11800.011.86491
CGGGCCC2150.00477946911.3958231
CGCCCCT6301.546141E-1010.5559931
GGCGCGC5108.232564E-810.29411338
CCGGGAT30450.010.23030951
GCCGAAT14750.09.9665161
CGGGAAT34300.09.7963261
GCCAGAT34250.09.7084311
CCGGGAC17850.09.6082421
GCCGGCT16600.09.4883461
GCCGGAT28300.09.2760041
CGGCAAT25050.09.221941
GTCGGAT45950.09.0645771
CCCCCCT4251.1496091E-49.0591991
CCCGCAT12800.09.0238121
CGGGGAT17900.08.7992481
CCGGCAT20300.08.7934691
CCCGGGG7655.2223186E-98.69317151